The present invention relates to a method for preparing a dermis layer-like structure by a three-dimensional cell printing technology, a cell culture structure prepared by the method, for example, a method for manufacturing a dermis layer-like structure or artificial skin, and a cell culture structure prepared by the method, for example, a dermis layer-like structure or artificial skin, and the artificial skin manufactured by the three-dimensional printing manufacturing method according to the present invention is useful for mass-production and can be used as a base technology for development of artificial skin model further closer to actual skin.
