Disclosed is an enzyme solution comprising a nonionic surfactant, an enzyme, a normal-temperature enzyme stabilizer, an isothiazolinone, a benzoisothiazolinone, sodium benzoate, a solubilizer, a pH adjuster, and water. In some embodiments, the enzyme solution also contains a chelating agent. The enzyme solution according to the present disclosure may be used to clean medical devices, and has good compatibility with non-woven fabrics comprising viscose fiber and terylene fiber. The enzyme solution dispersed in the non-woven fabric not only has good enzyme stability, but also has good mold-resistance and bacteria-resistance properties. Furthermore, the enzyme-containing non-woven fabric according to the present disclosure is less likely to cause damage to medical devices, and is less likely to cause corrosion to the metal or rubber components of the medical devices.