Disclosed herein, in certain embodiments, is an HC&bull；HA complex comprising hyaluronan and a heavy chain of I&alpha；I, wherein the transfer of the heavy chain of I&alpha；I is catalyzed by TSG-6. Further disclosed herein, in certain embodiments, is an HC&bull；HA complex comprising hyaluronan and a heavy chain of I&alpha；I, wherein the transfer of the heavy chain of I&alpha；I is catalyzed by the TSG-6 like protein. Additionally, disclosed herein are methods of manufacturing said complex and methods of use thereof.
