A biological fiber composite dressing, containing: a cloth membrane which has opposite a first surface layer and a second surface layer, and has cloth membrane fiber and a biological fiber membrane which has opposite a third surface layer and a fourth surface layer, and is formed by a bacterium of Gluconacetobacter, wherein the fourth surface layer is combined on the first surface layer, and a plurality of biological fibers being wound and combined on the cloth membrane are extended along the fourth surface layer of the biological fiber membrane, so that the moisturizing effects of the dressing can be enhanced through the biological fiber membrane, and carrying more active ingredients.一種生物纖維複合敷料,包括:具有相對之第一表層和第二表層的布膜,且該布膜具有布膜纖維;以及具有相對之第三表層和第四表層的生物纖維膜,係由葡糖醋桿菌屬(Gluconacetobacter)之菌所形成,且該第四表層係結合於該第一表層上,該生物纖維膜之第四表層延伸有複數纏繞結合在該布膜纖維之生物纖維,俾藉由該生物纖維膜提升該敷料的保濕性,並可攜帶更多活性成分。1‧‧‧生物纖維複合敷料10‧‧‧布膜10a‧‧‧第一表層10b‧‧‧第二表層12‧‧‧生物纖維膜12a‧‧‧第三表層12b‧‧‧第四表層