The invention relates to a device (7) for collecting particles and microorganisms present in ambient air, the device comprising a cyclone enclosure (8) for centrifuging air, the enclosure being of conical or frustoconical shape, external air inlet means (16) for admitting air into the enclosure (8), and air outlet means connected by coupling means (19, 20, 21) to air inlet filter means (4) of an individual motorized respiratory protection appliance (A).