PROBLEM TO BE SOLVED: To develop technology of separating and purifying a low molecular weight useful component with high purity, for example, a chlorogenic acid isomer from coffee, tea, green tea, cocoa or the like by a simple method.SOLUTION: An extraction liquid obtained from the raw material is separated and purified by high-speed countercurrent chromatography in which a solid phase and a mobile layer is fabricated using a solution of a composition of butanol, water and acetic acid.COPYRIGHT: (C)2012,JPO&INPIT【課題】コーヒー、紅茶、お茶、ココア等から簡便な方法で純度の高い低分子有用成分、例えばクロロゲン酸異性体を分離精製する技術の開発。【解決手段】前記原料から得た抽出液をブタノール/水/酢酸からなる組成の溶液を用いて固定相と移動層を作成し高速向流クロマトグラフィーにて分離精製する。【選択図】なし
