YEH, MEI LIANG,叶美良,葉美良,WEI, YU SHAN,魏钰珊,魏鈺珊,CHEN, WEI JEN,陈威仁,陳威仁
申请号：
TW103130054
公开号：
TW201610149A
申请日:
2014.09.01
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
The present invention provides a method of stably batch production of the Ophiocordyceps sinensis fruiting body, which includes utilization of the affinity gene sequencing analysis technology to sieving out the dominant strain of Hirsutella sinensis, which can fast and steadily produce the Ophiocordyceps sinensis fruiting body wherein the disclosed method is to inoculate the dominant strain into the artificial culture media containing the silkworm chrysalis extract, peptone, yeast extract, glucose, superfine sugar, food-grade CMC, trace element, food-grade agar powder, or agar gum. Through a temperature and humidity variation over daytime and nighttime and inducing growth of the fruiting body by the LED lamp, the industrialized scale batch production is achieved to produce the Ophiocordyceps sinensis fruiting body. Moreover, through the testing, the indicative ingredients of Ophiocordyceps sinensis fruiting body includes the polysaccharide, the Cordycepic acid, and the Adenosine, with all their contents being higher than those of the natural Ophiocordyceps sinensis fruiting body.本發明係提供一種穩定批次量產冬蟲夏草子實體的方法,包含利用親合性基因序列分析技術,來篩選中華被毛孢優勢菌株,而該優勢菌株可快速及穩定生產冬蟲夏草子實體;其中該方法係將該優勢菌株接種於包含蠶蛹萃取液、蛋白腖、酵母萃取物、葡萄糖、特砂、食品級羧甲基纖維素、微量元素、食品級寒天粉或洋菜膠之人工培養基中,經過日夜溫度與濕度變化,並以LED燈管照射誘導子實體生長,來達到工業化的規模,批次量產冬蟲夏草子實體。且經檢測,該人工冬蟲夏草子實體之指標性成分包括多醣、蟲草酸以及腺苷等含量,皆高於天然冬蟲夏草子實體。S11‧‧‧篩選菌株S12‧‧‧接種S13‧‧‧人工培養基培育S14‧‧‧可變溫度、濕度及光照環境中培養
