Provided are a hemoglobin-containing liposome having a specific membrane composition which secures high encapsulation efficiency of hemoglobin and exhibits excellent physical stability and in-vivo stability and a method for producing the hemoglobin-containing liposome. A hemoglobin-containing liposome includes a hemoglobin solution as an internal fluid of a liposome, in which the membrane of the liposome is constituted of a lipid mixture of a phospholipid, cholesterol, and a saturated higher fatty acid, and a molar ratio of the cholesterol to the phospholipid (cholesterol/phospholipid) is 0.7 to 1.0, and the content of stearic acid in the lipid mixture is 25 to 30 mol %. Preferably, the membrane of the liposome further contains a polyethylene glycol-bound phospholipid in an amount of 0.8 mol % or more relative to the total amount of the lipids constituting the membrane, and the polyethylene glycol-bound phospholipid is bound onto the outer surface of the membrane.
