A method for patient setup includes: obtaining a first two-dimensional movie having a first plurality of image frames； obtaining a first plurality of two-dimensional images； and displaying the first plurality of two-dimensional images together with the image frames from the first two-dimensional movie in an overlay configuration and in a synchronized manner, wherein the act of displaying is performed during a patient setup procedure. A method for patient setup includes: obtaining a plurality of three-dimensional images； obtaining a first two-dimensional movie having a first plurality of image frames； determining a first plurality of two-dimensional images from the plurality of three-dimensional images； and displaying the first plurality of two-dimensional images together with the image frames from the first two-dimensional movie in an overlay configuration and in a synchronized manner, wherein the act of displaying is performed during a patient setup procedure.
