Arthur David Laganowsky,David S. Eisenberg,Rebecca Anne Villarama
申请号：
US14373629
公开号：
US20150307573A1
申请日:
2013.01.22
申请国别(地区):
US
年份:
2015
代理人:
摘要：
This invention relates, e.g., to a cylindrin, which is a non-covalent assembly of substantially identical chains of an amyloid or amyloid-related protein, which is a non-covalent assembly of substantially identical chains of an amyloid or amyloid-related protein, wherein each chain has a length of about 10-100 amino acid residues and comprisesa single copy of a cylindrin-forming segment, ortandem adjacent copies of a cylindrin-forming segment, optionally separated by spacers, oradjacent copies of a first cylindrin-forming segment and a second complementary segment of the first cylindrin-forming segment, optionally separated by spacers,wherein at least about ⅔ of the amino acid residues in the chain are cylindrin-forming segments,wherein the cylindrin is a curved beta sheet formed from anti-parallel out-of-register extended protein strands, which is substantially filled with packed side chains.Also disclosed are methods for designing and making cylindrins, and methods for using them to identify inhibitors cylindrin-mediated cell toxicity.
