Disclosed herein is a method for manufacturing hemostatic fibers. The method includes the following steps: (a) mixing β-chitin and a base to obtain deacetylated β-chitin by a deacetylation reaction, in which the deacetylation degree of the deacetylated β-chitin is less than about 80% (b) acidifying the deacetylated β-chitin by an acid and (c) mincing the acidified and deacetylated β-chitin to obtain the hemostatic fibers.在此揭露一種止血纖維之製造方法,其包括以下步驟:(a)混合β-甲殼質與一鹼,以進行一去乙醯化反應,而得到一去乙醯化β-甲殼質,其中去乙醯化β-甲殼質之去乙醯度為低於約80%;(b)將去乙醯化β-甲殼質進行酸化處理;以及(c)將酸化處理後之去乙醯化β-甲殼質絞碎成纖維狀,而得到止血纖維。100...方法110、120、130...步驟