The Board of Trustees of the Leland Stanford Junior University
发明人:
Santiago Juan G.,Persat Alexandre,Garcia Giancarlo,Eid Charbel
申请号：
US201514828487
公开号：
US10132775(B2)
申请日:
2015.08.17
申请国别(地区):
美国
年份:
2018
代理人:
Molinelli Eugene J.`Beusse Wolter Sanks & Maire
摘要：
Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.
