VAINSTEIN ALEXANDER,MARTON IRA,HONIG ARIK,MARHEVKA ELENA
申请号:
IN201727022350
公开号:
IN201727022350A
申请日:
2017.06.27
申请国别(地区):
IN
年份:
2017
代理人:
摘要:
A nucleic acid construct is provided. The construct comprises a tobacco rattle virus (TRV) sequence and a nucleic acid sequence encoding a single guide RNA (sgRNA) that mediates sequence specific cleavage in a target sequence of a genome of interest wherein the TRV sequence is devoid of a functional 2b sequence. Also provided are plant cells comprising the construct and uses of the construct in gene editing.