The present invention relates to the methods for diagnosing TB in subject, which comprises (a) provides the sample from the subject, and the sample is selected from: the group of blood, serum and blood plasma composition (b) concentration of following biomarker in the sample: IL-1ra, IL6, IL-7, IL-8, IL-12p70, basic FGF, IP-10 and VEGF is determined (c) concentration of the every kind of biomarker determined in (b) is converted into tenths numerical value And (d) by the way that the tenths numerical value of (c) to be compared with following specific quantile cutoff value, each tenths numerical value is converted into binary system existence or non-existence, wherein, matching or will exist more than the tenths numerical value binary system that is converted to biomarker of specific quantile cutoff value, and the tenths numerical value that will be less than specific quantile cutoff value is converted to the binary system of biomarker and is not present The presence for wherein detecting every kind of biomarker shows the subject with TB. The present invention also relates to purposes, kit and equipment.