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Elimination of target cells by Cytotoxic T cells specific for viruses using MHC class i complexes that include
专利权人:
F. HOFFMANN-LA ROCHE AG
发明人:
申请号:
ARP120102174
公开号:
AR086982A1
申请日:
2012.06.19
申请国别(地区):
AR
年份:
2014
代理人:
摘要:
Herein is reported a complex comprising a First Component derived from an Antibody that specifically binds to a Target antigen, and as a Second Component a Peptide derived from a virus bound to a Protein Complex of MHC class I.The complex may be indicated in this memory Cytotoxic T Cells (circulating virus specific Memory T cells and effector T cells) of individual Cells that express the target antigen, which binds specifically to the part of the complex co Valente from an Antibody.Through the coverage of these cells with an MHC class i Complex mimicking an acute viral infection.In this way, a Point indicated in the present report is a complex, characterized in that it comprises a fusion polypeptide comprising, in the direction of n-terminal to c-terminal, B2 Microglobulin (i) and (ii) The extracellular Domains A1, A2 and A3 a MHC class i Molecule A relative frequency of 1% or greater, and two polypeptide Chains.We are united by one or more disulfide Bonds, in which the first polypeptide Chain Linked by Disulphide comprises, in the direction of n-terminal to c-terminal Domain, (i) a variable immunoglobulin Heavy or light chain, (ii) a constant domain of light chain or PE Sada immunoglobulin, and (iii) a polypeptide of Hinge region of heavy chain of antibodyAnd the second polypeptide Chain Linked by Disulphide polypeptide comprises a Hinge region of heavy chain Antibody, in which the fusion polypeptide is covalently attached to the c-terminus and n-terminus of the polypeptide Chains of the United Through DisulphideOr is covalently linked to the n-terminus of a variable Domain Antibody is the variable domain of heavy chain or complementary to the light within the first polypeptide Chain Linked by disulfide Bonds.Claim 1: Method for the recombinant production of a complex that includes: i) a fusion polypeptide of B2 Microglobulin and extracellular Domains A1, A2 and A3 a MHC class i Molecule, (ii) a couple of polypeptide Chains joined by a disulfide derivative Hinge re
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