A protective mechanism of heat inactivation to enhance Levilactobacillus brevis PDD-2 against alcohol-induced chronic liver disease based on proteomic analysis

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作   者：

Liu, Mingzhen;  Jiang, Xiaoxiao;  Zeng, Xiaoqun;  Guo, Yuxing;  Zhang, Tao;  Fan, Xiankang;  Xu, Jue;  Wu, Zhen;  Pan, Daodong; 

作者机构：

Ningbo Univ;  Nanjing Normal Univ; 

关键词：

TECHNOLOGIES;  MICE;  GAMMA-AMINOBUTYRIC-ACID;  GUT MICROBIOTA;  STRESS;  ACIDOPHILUS CICC 6074; 

期刊名称：

Food & Function

i s s n：

2042-6496

年卷期：

2024 年 15 卷 16 期

页   码：

8356-8369

页   码：

摘   要：

A proteomics-based analysis of the effect of heat inactivation on the alleviation of alcoholic liver disease (ALD) using Levilactobacillus brevis PDD-2 is presented, aimed at exploring the potential and mechanisms of postbiotic elements prepared through heat inactivation in the treatment of ALD. It was found that L. brevis PDD-2 and its postbiotic (heat-inactivated L. brevis PDD-2) alleviate chronic ALD via the gut-liver axis. In particular, heat-inactivated L. brevis PDD-2 significantly increased the relative abundance of Erysipelotrichaceae and better facilitated the oxidative stress balance in the liver. The tandem mass tag (TMT)-based quantitative proteomics technique analyses revealed that heat-inactivated L. brevis PDD-2 was associated with up-regulated expression levels of proteins related to the redox system, cellular metabolism, amino acid and oligopeptide transport, and surface proteins with immunomodulatory capacity. These findings provide a theoretical basis for developing novel therapeutic strategies and lay a solid foundation for further revealing its exhaustive mechanisms.