Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells

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作   者：

Kumar, Pranesh;  Rawat, Atul;  Keshari, Amit K.;  Singh, Ashok K.;  Maity, Siddhartha;  De, Arnab;  Samanta, Amalesh;  Saha, Sudipta; 

作者机构：

Dept Pharmaceut Technol;  Sanjay Gandhi Postgrad Inst Med Sci Compus;  Lucknow 226014;  Uttar Pradesh;  Babasaheb Bhimrao Ambedkar Univ;  Raebareli Rd;  India;  Kolkata 700032;  Jadavpur Univ;  Lucknow 226025;  Ctr Biomed Res;  Dept Pharmaceut Sci; 

关键词：

molecular docking;  caspase-3 and 8;  isoquinoline alkaloid;  Huh-7 cells; 

期刊名称：

Natural product research

i s s n：

1478-6419

年卷期：

2016 年 30 卷 4/6 期

页   码：

460-463

页   码：

摘   要：

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 mu M) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.