何健，籍贯江西南康，中国微生物学会环境微生物学术委员会委员、中国微生物学会资源微生物学术委员会委员、江苏省生物技术协会环境生物技术委员会副主任。南京农业大学“133重点人才工程”优秀骨干教师、江苏省“青蓝工程”优秀青年骨干教师培养对象、教育部新世纪优秀人才计划获得者。教学：基础微生物学，基础微生物学实验，现代微生物研究技术与方法。研究方向：环境微生物学与环境工程，除草剂微生物降解菌株、基因资源发掘和除草剂降解代谢机制研究。

研究方向

环境微生物学与环境工程，除草剂微生物降解菌株、基因资源发掘和除草剂降解代谢机制研究。

所在机构

名称：南京农业大学生命科学学院微生物学系 邮编：210095 地址：南京市南京卫岗1号

主要成就

近5年发表论文情况。 1)Chenghong Wang, Qing Chen, Rui Wang, Chao Shi, Xin Yan, Jian He*, Qing Hong, Shunpeng Li. A Novel Angular Dioxygenase Gene Cluster, Encoding 3-Phenoxybenzoate 1’, 2’ -Dioxygenase in Sphingobium wenxiniae JZ-1. Appl. Environ. Microbiol. 2014, 80(13):3811-3818. (* 通讯作者) 2)Qing Chen, Cheng-Hong Wang, Shi-Kai Deng, Ya-Dong Wu, Yi Li, Li Yao, Jian-Dong Jiang, Xin Yan, Jian He*, and Shun-Peng Li. A Novel Three-Component Rieske Non-Heme Iron Oxygenase 2 (RHO) System Catalyzing the N-Dealkylation of Chloroacetanilide Herbicides in Sphingomonads DC-6 and DC-2. Appl. Environ. Microbiol. doi:10.1128/AEM.00659-14. 3)Wang B, Guo P, Hang B, Li L, Jian He*, Li S. Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. Appl. Environ. Microbiol, 2009; 75(17): 5496-5500. 4)Hang Bao-Jian, Qing Hong, Xiang-Ting Xie, Xing Huang, Cheng-Hong Wang, Jian He*, Shun-Peng Li. SulE, a sulfonylurea herbicide de-esterification esterase from Hansschlegelia zhihuaiae S113.Appl. Environ. Microbiol, 2012; 78(6): 1962-1968. 5)ZhangJun, Jin-Gang Yin, Bao-Jian Hang, Shu Cai, Jian He*, Shun-Gui Zhou*and Shun-Peng Li. Cloning of a novel arylamidase gene from Paracoccus sp.FLN-7 that hydrolyzes amide pesticides. Appl. Environ. Microbiol,2012; 78(6):4848-4855. 6)Yong Yuan, Ya Tao, Shungui Zhou*, Tian Yuan, Qin Lu, Jian He*. Electron transfer capacity as a rapid and simple maturity index for compost. Bioresource Technology,2012; 116:428-434. 7)Zhang Jun; Jin-Wei Zheng; Bin Liang; Cheng-Hong Wang; Ying-Ying Ni; Shu Cai; Jian He*; Shun-Peng Li. Biodegradation of chloroacetamide herbicides by Paracoccus sp. FLY-8. J Agric Food Chem, 2011; 59(9): 4614-21. 8)Nie Zhi Juan, Hang Bao-Jian, Cai Shu, XIE Xiang-Ting, Jian He*, Li Shunpeng. Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1, and cloning of a novel gene encoding a CyB-hydrolyzing esterase. J Agric Food Chem, 2011; 59(9): 4614-21. 9)Guo P, Wang B, Hang B, Li L, Ali SW, Jian He*, Li S. Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase.Int Biodeter Biodegr. 2009; 63(8):1107-1112. 10)Lang Zhi-Fei, Jing-Jing Shen, Shu Cai, Juan Zhang, Jian He*and Shun-Peng Li. Expression, characterization and site-directedmutation of a multiple herbicide-resistant acetohydroxyacid synthase (rAHAS) from Pseudomonas sp. Lm10. Curr Microbiol,2011, 63(2): 145-150. 11)Wang Bao-zhan, Yun Ma, Wei-you Zhou, Jin-wei Zheng, Jian-chun Zhu, Jian He* and Shun-peng Li.Biodegradation of synthetic pyrethroids by Ochrobactrum tritici strain pyd-1. World J Microb Biot, 2011, 27: 2315-2324. 12)Wang Bao-Zhan, Peng Guo, Jin-Wei Zheng, Bao-Jian Hang, Lian Li, Jian He*, and Shun-Peng Li. Sphingobium wenxiniae sp. nov., a synthetic pyrethroids (SPs)-degrading bacterium isolated from activated sludge in a SPs-manufacturing wastewater treatment facility.Int J Syst Evol Microbiol, 2011, 61(8): 1776-1780. 13)Zhang Jun, Jin-Wei Zheng, Bao Jian Hang, Ying-Ying Ni, Jian He*, and Shun-Peng Li. Rhodanobacter xiangquanii sp. nov., a Novel anilofos-degrading bacterium isolated from a wastewater treating system. Curr Microbiol, 2011; 62: 645–649. 14)Li Lian, Jin-Wei Zheng, Bao-Jian Hang, Nina V. Doronina, Yuri A. Trotsenko, Jian He*, and Shun-Peng Li. Methylopila jiangsuensis sp. nov., a novel aerobic facultatively methylotrophic bacterium. Int J Syst Evol Microbiol, 2011, 61(8): 1561-1566. 15)Yu Xin-Yan, Yong-Feng Li, Jin-Wei Zheng, Yi Li, Lian Li, Jian He*, and Shun-Peng Li. Comamonas zonglianii sp. nov., isolated from phenol-contaminated soil. Int J Syst Evol Microbiol, 2011, 61(2): 255-258. 16)Zheng Jin-Wei, Yi-Guang Chen, Jun Zhang, Ying-Ying Ni, Wen-Jun Li, Jian He*, and Shun-Peng Li. Catellibacterium caeni sp. nov., and reclassification of Rhodobacter changlensis Anil Kumar et al. 2007 as Catellibacterium changlense comb. nov. Int J Syst Evol Microbiol, 2011, 61(8): 1921-1926. 17)Guo Peng, Bao-Zhan Wang, Bao-Jian Hang, Lian Li, Shun-Peng Li, and Jian He*. Sphingobium faniae sp. nov., a pyrethroid-degrading bacterium isolated from activated sludge treating wastewater from pyrethroid manufacture. Int J Syst Evol Microbiol, 2010(60): 408-412. 18)Zhang Jun, Shu-An Chen, Jin-Wei Zheng, Shu Cai, Bao-Jian Hang, Jian He*, and Shun-Peng Li. Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge of a wastewater bio-treating system, and emended description of the genus Catellibacterium. Int J Syst Evol Microbiol, 2012 (62): 495-499. 19)Zhang Jun, Jin-wei Zheng, Byung Cheol Cho, Chung Yeon Hwang, Chengxiang Fang*, Jian He* and Shun-Peng Li. Sphingobacterium wenxiniae sp. nov., a novel cypermethrin-degrading bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbio, 2012(62): 683-687. 20)Zhang Jun, Zhi-Fei Lang, Jin-Wei Zheng, Bao-Jian Hang, Xiao-Qin Duan, Jian He*, and Shun-Peng Li. Sphingobium jiangsuense sp. nov., an m-phenoxybenzoic acid-degrading bacterium isolated from a wastewater treating system.Int J Syst Evol Microbio, 2012(62): 800-805. 21)Chen B, Xin Huang, Jin-Wei Zheng, Shun-Peng Li, Jian He*. Candida mengyuniae sp. nov., a metsulfuron-methyl-resistant yeast. Int J Syst Evol Microbiol, 2009(59):1237-1241. 22)Zhi-Fei Lang, Jing-Jing Shen, Shu Cai, Juan Zhang, Jian He*and Shun-Peng Li. Expression, characterization and site-directedmutation of a multiple herbicide-resistant acetohydroxyacid synthase (rAHAS) from Pseudomonas sp. Lm10. Curr Microbiol (IF 1.33),2011(63): 145-150. 9 23)Li-Na Sun, Jun Zhang, Soon-Wo Kwon, Jian He*, Shun-Gui Zhou, and Shun-Peng Li Paracoccus huijuniae sp. nov., an amide pesticides-degrading bacterium isolated from activated sludge of a wastewater bio-treating system Int J Syst Evol Microbiol,2013(63): 1132-1137. 24)Yi Li, Jun Zhang, Qing Chen, Guiqing Yang, Shu Cai, Jian He*, Shungui Zhou, and Shun-Peng Li. Dokdonella kunshanensis sp. nov., isolated from activated sludge and emended description of the genus Dokdonella. Int J Syst Evol Microbiol,2013(63): 1519-1523. 25)Li-Na Sun, Jun Zhang, Qing Chen, Jian He*, Qin-Fen Li, and Shun-Peng Li. Comamonas jiangduensis sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil. Int J Syst Evol Microbiol. 2013, 63, 2168–2173. 26)Li-Na Sun, Jun Zhang, Qing Chen, Jian He*, and Shun-Peng Li. Sphingobacterium caeni sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol. ijs.0.046987-0; published ahead of print November 12, 2012.2013 Jun;63(Pt 6):2260-2264. 27)Shi-Kai Deng, Xiao-Mei Ye, Cui-Wei Chu, Jin Jiang, Jian He*, Jun Zhang, and Shun-Peng Li. Chryseomicrobium aureum sp. nov., a bacterium isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 2014, DOI 10.1099/ijs.0.061143-0 28)Qing Chen, Jun Zhang, Cheng-Hong Wang, Jin Jiang,Soon-Wo Kwon, Li-Na Sun,Wen-Biao Shen and Jian He*. Novosphingobium chloroacetimidivorans sp. nov., a chloroacetamide herbicide–degrading bacterium isolated from activated sludge. Int. J. Syst. Evol. Microbiol. (2014), 64, 000–000 DOI 10.1099/ijs.0.062950-0. 29)沈晶晶, 李永丰, 黄星, 俞欣妍, 何健*, 李顺鹏. 乙酰羟酸合成酶同工酶基因ilvBN、ilvGM 和ilvIH 的表达及对除草剂抗性的比较. 生物工程学报, 2009, 25(7): 1007-1013. 30)邱吉国, 郑金伟, 张隽, 李顺鹏, 何健*. 乙羧氟草醚降解菌Pseudomonas sp. YF1的分离、鉴定与降解特性. 应用与环境生物学报, 2009, 15 (5): 686~691. 31)张隽, 黄星, 邱吉国,何健*. 氟铃脲降解菌FLN-1的分离鉴定及降解特性. 环境科学学报, 2009, 29 (6): 1178-1183. 32)李恋，王保战，周维友，杭宝剑，郭鹏，何健*，李顺鹏. Sphingobium sp. JZ-1对菊酯类农药的降解特性研究. 土壤学报, 2011, 48(2): 389-396. 33)李恋, 郑金伟, 杭宝剑, 王保战, 何健*, 李顺鹏. 菊酯类杀虫剂降解菌JZL-3 (Arthrobactersp.)的分离鉴定与降解特性研究. 安全与环境学报， 2010, 10(6): 63-67. 34)段晓芹, 郑金伟, 张隽, 杭宝建, 何健*, 李顺鹏. 3-苯氧基苯甲酸降解菌BA3的降解特性及基因工程菌的构建. 环境科学学报, 2011, 32(1): 240-246. 35)孙笑非, 黄星, 陈博, 李顺鹏, 何健*. 抗甲磺隆假单胞菌的分离及其乙酰乳酸合酶的大小亚基ilvIH 基因的克隆和表达. 微生物学报, 2008, 48(11): 1493~1498. 36)沈晶晶黄星俞欣妍李顺鹏，何健*. 两株抗乙酰羟酸合酶类除草剂克雷伯氏菌的分离及其AHAS基因ilvIH的克隆. 应用与环境生物学报, 2008, 14 (5): 663~667. 37)倪盈盈, 郑金伟, 王保站, 张隽, 李顺鹏, 何健*.酰胺类除草剂降解菌的分离、鉴定及降解特性. 应用环境生物学报, 2011, 17(05): 711-716. 38)郎志飞, 俞欣妍, 黄星，张隽, 何健*. Pseudomonas sp. L19抗除草剂AHAS基因克隆、表达和特性研究. 应用环境生物学报2012, 18(01): 49-54. 39)陈青，姚利，王成红，邓诗凯，褚翠伟，何健*. 乙草胺降解菌Sphingomonas sp. DC-6的分离鉴定及其代谢途径的初步研究. 中国农业科技导报，2013,15(5):67-74. 近3年获得专利： 1）乙酰羟酸合酶抑制剂类除草剂抗性基因ilvBN，专利号：ZL 200910029119，申请人：何健;李顺鹏; 沈晶晶; 黄星; 聂志娟； 2）降解乐果、氯苯胺灵和敌稗的酰胺酶基因dimtH及其编码的蛋白质及其应用，专利号： ZL201110208381.9, 申请人：何健; 李顺鹏; 张隽; 殷金岗; 杭宝剑； 3）酰胺酶基因 cmeH 及其编码蛋白质及其应用，专利号：ZL201210227768.3, 申请人：何健; 李怡; 陈青； 4）噻吩磺隆水解酶基因tsmE及其应用，专利号：ZL20111020744.2, 申请人：李顺鹏; 何健; 杭宝剑；谢香庭 5）拟除虫菊酯类杀虫剂水解酶基因，专利号：ZL200810243899.4, 申请人：李顺鹏; 何健; 王保战; 郭鹏; 李恋; 杭宝剑； 6）一种氯代乙酰胺类除草剂降解菌及其生产的菌剂和应用，申请号：201210228772.1, 申请人：何健; 李怡; 陈青； 7）一种加氧酶基因caceO及其编码的蛋白和应用，专利申请号：201310182539.9，申请人：何健; 陈青; 李怡。 8) 国际专利：THIFENSULFURON HYDROLASE GENE tsmE AND USES THERE OF 国际申请号：PCT/CN2012/078765. 国际公布号：WO 2013/013585 A1 李顺鹏，何健，杭宝建，谢香庭 9) 国际专利：CHLOROACETANILIDE HERBICIDES GENE CACEOAND USES THERE OF. 国际申请号：PCT/CN2014/077431. 何健，陈青，李怡，闫新，蒋建东，洪青，黄星，李顺鹏，朱建春