Cloning of Camellia sinensis MLO1 gene and analysis of disease resistance of miRNA as green pesticide
茶树MLO1基因的克隆及绿色农药miRNA的抗病性分析
- 关键词：
- 来源：
- Plant Cell, Tissue and Organ Culture 期刊
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2025-03-25
- 摘要：
- Tea plants (Camellia sinensis (L.) O. Ktze), a key economic crop in China, are severely impacted by diseases, significantly reducing tea production. Due to issues such as resistance and pesticide residues associated with traditional chemical control, developing green control methods is crucial. The Mildew Resistance Locus O (MLO) gene, a negative regulator of plant disease resistance, has been a focal point in plant disease resistance research for years. This study identified 28 CsMLO gene family members in the tea plant genome and selected the CsMLO1 gene, which exhibits significant expression changes under pathogen stress, for cloning and functional validation. The virus-induced gene silencing (VIGS) assay revealed that silencing CsMLO1 in tea plants significantly enhances their disease resistance. Further overexpression of the CsMLO1 gene in tobacco and subsequent infection with powdery mildew revealed a significant decrease in resistance upon overexpression. These results suggest that CsMLO1 negatively regulates plant disease resistance. Through database comparison analysis, we screened out the miRNA interacting with the CsMLO1 gene, namely novel-miRNA-76-5p. To investigate its function, we subjected the sprayed plants to pathogen stress experiments. The findings from the analysis indicated that the activities of various protective enzymes in tea leaves treated with novel miRNA-76-5p were significantly increased, the area of disease spots was significantly reduced, and the plant stress resistance was significantly enhanced. The experiments demonstrated that miRNA exhibits a significant control effect against fungal diseases in tea plants, offering a novel research direction for the eco-friendly management of tea plant diseases.
- 所属专题：
- 60
